Degradation Potential of Protocatechuate 3,4-Dioxygenase from Crude Extract of Stenotrophomonas maltophilia Strain KB2 Immobilized in Calcium Alginate Hydrogels and on Glyoxyl Agarose

Table 2

Effect of aliphatic alcohols on the activity of immobilized protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2. Data shown represent the average of three independent trials ± standard deviation.

Compound	Concentration, (𝜇M)	Relative activity of free enzyme, %	Relative activity of the enzyme immobilized in calcium alginate, %	Relative activity of the enzyme immobilized on glyoxyl agarose, %
None		100.0 ± 0.00	100.0 ± 0.00	100.0 ± 0.00
Methanol	100	96.53 ± 0.54	110.70 ± 6.88	37.07 ± 17.47
	200	91.53 ± 2.18	90.08 ± 2.48	45.17 ± 9.28
	300	101.54 ± 2.18	74.71 ± 2.2	100.00 ± 13.65
Ethanol	100	144.61 ± 13.06	90.47 ± 3.58	67.18 ± 14.20
	200	156.69 ± 3.81	116.54 ± 1.92	52.51 ± 5.46
	300	141.92 ± 1.63	155.65 ± 22.01	50.58 ± 28.94
Propanol	100	160.78 ± 22.84	100.39 ± 4.40	93.82 ± 9.28
	200	134.23 ± 5.98	101.75 ± 7.98	78.38 ± 8.19
	300	130.38 ± 1.63	101.75 ± 4.13	76.83 ± 11.47
Butanol	100	112.69 ± 1.63	122.28 ± 0.83	108.49 ± 3.82
	200	120.77 ± 2.18	118.10 ± 11.28	86.87 ± 30.03
	300	120.38 ± 2.72	109.15 ± 20.64	76.44 ± 0.0