Degradation Potential of Protocatechuate 3,4-Dioxygenase from Crude Extract of Stenotrophomonas maltophilia Strain KB2 Immobilized in Calcium Alginate Hydrogels and on Glyoxyl Agarose

Table 3

Effect of metals on the activity of immobilized protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2. Data shown represent the average of three independent trials ± standard deviation.

Compound	Concentration, (mM)	Relative activity of free enzyme, % [6]	Relative activity of the enzyme immobilized in calcium alginate, %	Relative activity of the enzyme immobilized on glyoxyl agarose, %
None		100.0 ± 0.00	100.0 ± 0.00	100.0 ± 0.00
Co^2+	1	74.34 ± 19.14	39.68 ± 8.98	61.65 ± 8.88
	2	70.58 ± 8.45	70.63 ±7.86	67.27 ± 0.00
	3	51.55 ± 4.70	97.62 ± 3.37	86.11 ± 9.82
Cu^2+	1	41.81 ±4.07	30.16 ± 0.00	85.12 ± 0.00
	2	27.36 ± 2.61	28.57 ± 2.24	80.16 ± 5.61
	3	29.64 ± 0.63	22.22 ± 0.00	85.12 ± 0.00
Ni^2+	1	78.65 ± 3.02	70.27 ± 0.00	69.58 ± 0.00
	2	88.34 ± 2.29	84.24 ± 3.84	44.63 ± 10.05
	3	83.85 ± 0.31	63.51 ± 11.47	59.67 ± 0.93
Mn^2+	1	108.06 ± 8.19	50.40 ± 1.13	70.74 ± 0.70
	2	98.55 ± 5.08	20.00 ± 3.39	75.87 ± 6.76
	3	80.92 ± 5.43	29.60 ± 3.39	83.30 ± 2.10
Cd^2+	1	69.33 ± 4.01	106.76 ± 7.64	84.70 ± 7.03
	2	63.85 ± 2.13	115.54 ± 8.60	112.62 ± 6.49
	3	60.20 ± 12.64	62.16 ± 3.82	93.12 ± 8.11
Al^3+	1	89.99 ± 5.43	46.62 ± 10.51	87.76 ± 4.33
	2	68.39 ± 3.21	83.11 ± 0.96	78.97 ± 9.19
	3	33.50 ± 2.14	131.08 ± 11.47	91.20 ± 17.31
Zn^2+	1	94.27 ± 7.21	27.20 ± 11.31	70.74 ± 0.70
	2	71.79 ± 5.17	11.20 ± 6.79	82.97 ± 3.97
	3	50.95 ± 2.76	4.80 ± 0.00	67.76 ± 9.58
Fe^3+	1	38.93 ± 6.17	180.16 ± 19.08	61.50 ± 0.00
	2	31.17 ± 6.08	150.79 ± 9.00	56.03 ± 6.54
	3	22.76 ± 2.11	141.27 ± 6.73	54.38 ± 5.61