Degradation Potential of Protocatechuate 3,4-Dioxygenase from Crude Extract of Stenotrophomonas maltophilia Strain KB2 Immobilized in Calcium Alginate Hydrogels and on Glyoxyl Agarose

Table 4

Effect of chelators on the activity of immobilized protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2. Data shown represent the average of three independent trials ± standard deviation.

Compound	Concentration, (𝜇M)	Relative activity of free enzyme, %	Relative activity of the enzyme immobilized in calcium alginate, %	Relative activity of the enzyme immobilized on glyoxyl agarose, %
None		100.0 ± 0.00	100.0 ± 0.00	100.0 ± 0.00
EDTA	100	135.07 ± 1.86	113.13 ± 7.11	46.72 ± 27.85
	200	128.19 ± 23.46	97.54 ± 3.91	30.89 ± 6.55
	300	18.19 ± 0.12	116.92 ± 8.35	32.43 ± 3.28
2,2′-dipyridyl	100	119.78 ± 0.87	123.81 ± 6.58	57.91 ± 0.00
	200	99.30 ± 0.99	111.62 ± 3.55	30.12 ± 17.47
	300	87.62 ± 4.85	117.40 ±3.56	22.01 ± 12.56
Phenanthroline	100	97.78 ± 7.33	117.53 ± 7.64	101.93 ± 1.09
	150	81.46 ± 4.10	121.04 ± 5.87	60.62 ± 16.93
	200	69.69 ± 3.11	101.56 ± 6.76	21.62 ± 7.64