Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis
Figure 4
DTH response induced by PPD-B, C1, and C2 in experimentally M. bovis-infected cattle to 90 dpi (). The cocktails were prepared with 10 μg of each antigen per 0.1 mL in PBS and PPD-B 100 μg per 0.1 mL. The animals were intradermally injected with 0.1 mL of C1, C2, or PPD-B (1 mg/mL) and the thickness of the caudal fold tuberculin skin test was measured using callipers prior and 72 h after injection. Animal responses were represented by boxes and the horizontal line provides the median with standard errors. The statistical difference between responses was determined by using Kruskall Wallis test ().