Effects of Stress and MDMA on Hippocampal Gene Expression
Table 4
(a) Genome GO:0051082 (unfolded protein binding). (b) Real-time PCR array of chaperone and heat shock genes.
(a)
Entrez ID
Symbol
Gene name
Average intensity
Stress versus no stress
MDMA versus no MDMA
No MDMA
MDMA
No stress
Stress
−Fold change
value
−Fold change
value
−Fold change
value
−Fold change
value
361384
Dnajb1
DnaJ (Hsp40) homolog, subfamily B, member 1
363.16
1.02
0.81
−1.09
0.50
2.18
0.00
1.96
0.00
65028
Dnaja1
DnaJ (Hsp40) homolog, subfamily A, member 1
1222.76
1.04
0.43
1.03
0.51
1.45
0.00
1.44
0.00
295549
Dnajb4
DnaJ (Hsp40) homolog, subfamily B, member 4
668.67
1.02
0.73
1.01
0.87
1.42
0.00
1.40
0.00
300721
Dnaja4
DnaJ (Hsp40) homolog, subfamily A, member 4
232.20
1.07
0.48
−1.10
0.21
1.39
0.01
1.18
0.01
288620
Cct6a
chaperonin subunit 6a (zeta)
395.91
1.03
0.73
−1.02
0.50
1.37
0.00
1.30
0.00
360734
Dnajb11
DnaJ (Hsp40) homolog, subfamily B, member 11
472.49
1.01
0.93
−1.02
0.75
1.36
0.00
1.33
0.00
29345
Serpinh1
Serine (or cysteine) peptidase inhibitor, clade H, member 1
469.00
−1.04
0.77
−1.12
0.47
1.36
0.15
1.26
0.00
295230
Cct3
chaperonin subunit 3 (gamma)
686.33
1.03
0.62
−1.06
0.26
1.35
0.00
1.24
0.00
311456
Mkks
McKusick-Kaufman syndrome protein
320.40
1.06
0.33
1.02
0.81
1.35
0.00
1.30
0.00
64202
Calr
Calreticulin
1882.72
1.03
0.63
−1.05
0.29
1.32
0.00
1.22
0.00
63868
Hspd1
Heat shock protein 1 (chaperonin)
1509.64
1.01
0.83
−1.00
0.98
1.28
0.01
1.26
0.00
29374
Cct4
Chaperonin subunit 4 (delta)
662.36
1.03
0.63
−1.04
0.45
1.28
0.00
1.20
0.00
25719
Scg5
Secretogranin V
2031.48
1.05
0.34
−1.00
0.94
1.26
0.00
1.19
0.00
362862
Tra1
Tumor rejection antigen gp96
2797.04
1.03
0.63
1.04
0.33
1.25
0.01
1.26
0.00
24818
Tcp1
t-complex protein 1
474.45
1.02
0.80
−1.01
0.81
1.24
0.00
1.20
0.01
301252
Hsp90ab1
Heat shock protein 90 kDa alpha (cytosolic), class B member 1
4755.12
1.01
0.72
−1.03
0.35
1.24
0.00
1.18
0.00
24468
Hspa8
Heat shock protein 8
3661.99
1.02
0.84
1.06
0.33
1.22
0.03
1.27
0.00
294864
Cct5
Chaperonin subunit 5 (epsilon)
1223.73
1.04
0.45
1.01
0.82
1.22
0.00
1.18
0.00
368044
Atp6v1g2
ATPase, H+ transporting, V1 subunit G isoform 2
1494.80
1.02
0.72
−1.03
0.38
1.22
0.00
1.15
0.00
294236
Gtf2h4
General transcription factor II H, polypeptide 4
106.29
1.08
0.39
−1.04
0.36
1.21
0.01
1.08
0.22
84026
Dnaja2
DnaJ (Hsp40) homolog, subfamily A, member 2
1040.62
1.05
0.47
1.06
0.06
1.21
0.00
1.23
0.00
619393
Dnajc12
DnaJ (Hsp40) homolog, subfamily C, member 12
367.66
1.06
0.29
−1.01
0.72
1.21
0.00
1.12
0.03
89811
Vegfb
Vascular endothelial growth factor B
330.14
−1.03
0.66
−1.04
0.33
−1.22
0.01
−1.23
0.00
299313
Uxt
Ubiquitously expressed transcript
616.75
−1.05
0.51
1.04
0.20
−1.29
0.00
−1.19
0.00
29427
Aif1
Allograft inflammatory factor 1
172.49
−1.07
0.47
−1.25
0.00
−1.39
0.00
−1.62
0.00
Individual members of the gene ontology category Unfolded Binding Protein are shown. The average intensity of all genes shown is above threshold. Fold changes and values beyond the cutoff for significance are in bold. For clarity, genes that do not reach significance in any category are not shown. Genes induced by MDMA are separated by a free line.
(b)
Gene symbol
Fold regulation
Hspa5
2.6069
Cct5
2.5859
Dnajb1
2.3823
Cryab
2.3305
Hspb1
2.2668
Hspa2
2.215
Bag3
2.0596
Dnaja4
1.8908
Cct7
1.8412
Dnajb11
1.8159
Hspa14
1.8117
Dnajb4
1.7724
Ldha
1.7399
Dnaja1
1.6982
Hsph1
1.6806
Dnajc7
1.6537
Hsp90ab1
1.5955
Cct6a
1.5808
Hsp90aa1
1.5609
Cct2
1.5112
Hsp90b1
1.5025
Serpinh1
1.4973
Dnaja2
1.4346
Hspa4l
1.4198
Real-time PCR validation of chaperone and heat shock gene upregulation by MDMA exposure (2 rats) versus unstressed untreated rats (3 rats). Fold change (2−ΔΔCt) is the normalized gene expression (2−ΔCt) in the test sample divided by the normalized gene expression (2−ΔCt) in the control sample. Fold regulation represents fold change results in a biologically meaningful way. Fold change values greater than one indicate a negative or downregulation and the fold regulation is the negative inverse of the fold change. Shown are positive changes in fold regulation by MDMA of at least 1.4 over controls.
