Epigallocatechingallate Inhibits Migration of Human Uveal Melanoma Cells via Downregulation of Matrix Metalloproteinase-2 Activity and ERK1/2 Pathway
Figure 3
Effect of EGCG on the secretion, protein, and mRNA expression of MMP-2. M17 cells were treated with various concentrations (0, 20, 40, 60, 80, and 100 μM) of EGCG for 24 hours. (a) The conditioned media were collected and the activity of MMP-2 was detected. (b) A gelatin zymography and western blot were performed for cell lysates. (c) Semiquantitative RT-PCR was performed to compare MMP-2 mRNA levels. (d) The mRNA levels of MMP-2 were quantified using a real-time PCR assay. The values represented the means ± SD of at least three independent experiments. as compared with the control.
