Epigallocatechingallate Inhibits Migration of Human Uveal Melanoma Cells via Downregulation of Matrix Metalloproteinase-2 Activity and ERK1/2 Pathway
Figure 6
Effect of U0126 and EGCG on the expression of MMP-2, TIMP-2, and ERK1/2 pathway and cell migration. M17 cells were pretreated with U0126 (10 μM) for 1 hour and then incubated in the presence or absence of EGCG (60 or 100 μM) for 24 hours. (a) The culture media were used as subjects for analysis of MMP-2 activity. (b) The cell lysates were used as subjects for analysis of MMP-2, TIMP-2, and RECK protein levels. (c) Cells were used for wound-healing assay as described in Materials and Methods section. The values represented the means ± SD of at least three independent experiments. as compared with the control. as compared with the EGCG-treated only.
