Jacobsen Catalyst as a Cytochrome P450 Biomimetic Model for the Metabolism of Monensin A
Figure 2
(a) Effect of monensin A (1 μM), metabolite 1 (1 μM), and metabolite 2 (1 μM) on the dissipation of the mitochondrial membrane potential. (b) Effect of monensin A (1 μM), metabolite 1 (1 μM), and metabolite 2 (1 μM) on mitochondrial swelling. (c) Effect of monensin A (1 μM), metabolite 1 (1 μM), and metabolite 2 (1 μM) on mitochondrial production of free radicals. All experiments were performed in mitochondria isolated from rat liver (1.0 mg protein·mL−1) incubated as described in Section 2. Points represent the mean ± SEM of three determinations with different mitochondrial preparations, relative to the control in the absence of the compound. *Different letters represent significant differences between treatments according to Tukey’s test ().
