Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes

Table 1

Nucleotide sequences of the selected unique probes and complementary zipcodes.

Probe name^1	Probe sequence^2        czipcode sequence^3	Pos.
Hybrid control	gttaccgctggtgctgccgccggta	66
Synth template	agccgcgaacaccacgatcgaccggcgcgcgcagctgcagcttgctcatg
DS synth template	catgagcaagctgcagctgcgcgcg
CP synth template	ccggtcgatcgtggtgttcgcggctgtggtgtgccagccgtcggtgccat	63
DS Morganella	ggcgtaaagcgcacgcaggcggttgattg
CP Morganella	agtcagatgtgaaatccccgggcttaacccgggatgtcagtgacgcgctcagcgttg	29
DS Shewanella	gatgtctactcggagtttggtgtcttgaacactgggc
CP Shewanella	tctcaagctaacgcattaagtagaccgcctggggagccgtacccttccgctggagatttac	23
DS Aeromonas	gccccgggctcaacctgggaattgcatttaaaactgt
CP Aeromonas	ccagctagagtcttgtagaggggggtagaattccagtgtgcgcccgagatcggtatccccg	17
DS Pseudomonas	cccttgtccttagttaccagcacgtiatggtgggc
CP Pseudomonas	actctaaggagactgccggtgacaaaccggaggggattgcaccgtcagcaccaccgag	14

1DS: discriminating probe; CP: common probe; ²discriminating positions are indicated as bold; ³complemantary zipcode sequences are underlined.