Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22
Figure 5
pH activity (a) and pH stability (b) of P. stutzeri crude enzyme. Amylolytic activity was assayed in the pH range of 3.0 to 13.0 at 60°C. The maximum activity obtained at pH 8.0 was considered as 100% activity. To assess the pH stability, the P. stutzeri crude enzyme was preincubated at the indicated pH at 30°C for 60 min and the residual enzyme activity was determined at pH 8.0 and 60°C. The activity of the crude enzyme before incubation was taken as 100%. Buffer solutions used for pH activity and stability are presented in Materials and Methods.
