Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22
Table 6
Time course of maltooligosaccharides production from 1% soluble starch by P. stutzeri crude enzyme.
Hydrolysis time
Oligosaccharides concentration (g/L) and percentage (%)
G1
G2
G3
G4
G5
G6
0 min
0
0
0
0
0
0
5 min
0
0
0
2.68 ( )
0
0
15 min
0
0.06 (1.1)
0.03 (0.5)
5.31 ( )
0.02 (0.4)
0
30 min
0
0.08 (1.6)
0.05 (0.95)
5.1 ( )
0.023 (0.45)
0
1 h
0
0.04 (0.8)
0.03 (0.8)
4.9 ( )
0.02 (0.4)
0.01 (0.2)
2 h
0
0.058 (1.2)
0.03 (0.6)
4.75 (97.5)
0.02 (0.41)
0.014 (0.29)
6 h
0
0.07 (1.4)
0.05 ( )
4.92 ( )
0.04 (0.8)
0.02 (0.4)
0.1 unit of the α-amylase activity was reacted with 1% of soluble starch at 60°C and pH 8.0 and reaction products were analyzed at different time intervals.
