Osthole Attenuates Doxorubicin-Induced Apoptosis in PC12 Cells through Inhibition of Mitochondrial Dysfunction and ROS Production

Figure 3

The effect of osthole on DOX-induced cytotoxicity in PC12 cells. (a) Cells were pretreated with different concentrations of osthole 2 h before exposure to 5 μM of DOX. Data are expressed as the mean ± S.E.M of three separate experiments. ^##𝑃<0.01 versus control, ^∗𝑃<0.05 versus DOX-treated cells. (b) Representative photomicrograph shows morphological changes of cells. Cells were pretreated with osthole (7 μg/cc) for 2 h before exposure to 5 μM of DOX and imaged by inverted phase contrast microscope.

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