Osthole Attenuates Doxorubicin-Induced Apoptosis in PC12 Cells through Inhibition of Mitochondrial Dysfunction and ROS Production

Figure 7

Effect of osthole on DOX-induced ROS overproduction. Cell pretreated with osthole 2 h before exposure to 5 μM of DOX. The fluorescence intensity of DCF was measured in a microplate reader at an excitation wavelength of 488 nm and an emission wavelength of 535 nm. The data were represented as mean ± S.E.M for three independent experiments. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 versus control group. ^#𝑃<0.05 and ^##𝑃<0.01 versus group treated with DOX alone.