The Early Activation of CD8⁺ T Cells Is Dependent on Type I IFN Signaling following Intramuscular Vaccination of Adenovirus Vector

Figure 2

Relative mRNA expressions in the iLNs and MLN of WT and IFNAR2 KO mice following i.m. Adv vaccination. At 8 hours after the i.m. vaccination of 10¹⁰ vp of Ad-LacZ, total RNA was extracted from mononuclear cells in the LNs of each mouse. The mRNA expressions of Ifna, Ifnb, Il1b, Tnfa (a), Mb21d1 (b), Tlr9, and Ddx41 (c) in the LNs were measured by qRT-PCR, normalized by Actb. The graphs represent the relative mRNA expression of each gene normalized by that of PBS-administrated WT mice. Data are shown as the means ± S.E.M. (𝑛=3) and are representative of two independent experiments.^∗𝑃<0.05 compared with other groups except for the MLN of Adv-administrated WT mice.^∗∗𝑃<0.01 compared with the iLNs of IFNAR2 KO mice. ^#𝑃<0.0001 compared with other groups.

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