BioMed Research International / 2014 / Article / Tab 3

Review Article

N-Acyl Homoserine Lactone-Mediated Quorum Sensing with Special Reference to Use of Quorum Quenching Bacteria in Membrane Biofouling Control

Table 3

The biosensors strains developed to detect AHLs produced by Gram-negative bacteria.

Biosensor strain/plasmidResponded AHLsReporter systemReference

Ag. tumefaciens NT1 (pDCI41E33 containing traG::lacZ fusion)C6-HSL, C8-HSL, C10-HSL, C12-HSL, C14-HSL, and AHLs with 3-oxo-, 3-hydroxy-, and
3-unsubstituted side chains
-Galactosidase activity[94]

Ag. tumefaciens NT1 (pZLR4 containing Tral/R)C6-HSL, C8-HSL, C10-HSL, C12-HSL, C14-HSL, 3-hydroxy-C6-HSL, 3-hydroxy-C8-HSL, 3-hydroxy-C10-HSL, and all AHLs with 3-oxo-side chains -Galactosidase activity[95]

Ag. tumefaciens A136 (traI-lacZ fusion (pCF218) (pCF372))C6-HSL, C8-HSL, 3-oxo-C8-HSL, C10-HSL, C12-HSL, 3-oxo-C12-HSL, and C14-HSL -Galactosidase activity[37]

C. violaceum CV026 (CviVR receptor)C4-HSL, C6-HSL, and C8-HSLViolacein pigmentation[53, 96]

C. violaceum VIR24 (CviI receptor)3-Oxo-C6-HSL, C6-HSL, C7-HSL, 3-oxo-C8-HSL, C8-HSL, C10-HSL, C12-HSL, and C14-HSL Violacein pigmentation[97]

E. coli (luxCDABE cassette activated by Ahyl/R of A. hydrophila) C4-HSLBioluminescence[45]

E. coli (pSB401 containing LuxI/R of V. fischeri)C6-HSL
3-Oxo-C8-HSL
C8-HSL
luxCDABE[98]

E. coli (pHV2001 containing luxI/R)C6-AHL
C8-3-oxo-HSL
C8-HSL
luxCDABE
[99]

E. coli (pSB1075 containing LusI/R of P. aeruginosa)3-Oxo-C12-HSL, C12-HSLluxCDABE[98]

E. coli (pHV2001-containing LuxI/R of V. fischeri)C6-HSL, 3-oxo-C6-HSL, 3-oxo-C8-HSL, and C8-HSL luxCDABE[100]

E. coli (pKDT17 containing LusI/R of P. aeruginosa)3-Oxo-C10-HSL, C10-HSL, 3-oxo-C12-HSL, and C12-HSL -Galactosidase activity[100]

P. putida 117 (pAS-C8-CepR receptor)C8-HSLBioluminescence[101]

P. aeruginosa (M71LZ containing Lasl/R)3-Oxo-C10-HSL, 3-oxo-C12-HSL -Galactosidase activity[102]

P. aeruginosa (pSB406 containing RhlI/R)C4-HSL, C6-HSL, C8-HSL, C10-HSL, C12-HSL, and C14-HSL with 3-oxo-side chainsluxCDABE[98]

P. fluorescens (pSF105 + pSF107 containing Phzl/R)3-Hydroxy-C6-HSL, 3-hydroxy-C8-HSL -Galactosidase activity[103]

Si. meliloti Rm41 (sinI::lacZ pJNSinR)C14-HSL, 3-oxo-C14-HSL, C16-HSL, and 1-3-oxo-C16-HSL -Galactosidase activity[104]

V. fischeri (pJBA88 and pJBA89 encoding luxR and Pluxl fusion of gfpmut3*)C6~C14-3-oxo-HSL
C6~C12-HSL
gfp[105]