Trichostatin A Suppresses EGFR Expression through Induction of MicroRNA-7 in an HDAC-Independent Manner in Lapatinib-Treated Cells

<table>Proteasomal or lysosomal protein degradations were not involved in TSA-induced EGFR downregulation. 231/Lap#6 cells were pretreated with proteasomal inhibitor 5 <i>μ</i>M PSI, 5 <i>μ</i>M lac, and 1 <i>μ</i>M MG132 for 2 hrs (a) or with lysosomal inhibitor 1 <i>μ</i>M NH<sub>4</sub>Cl and 25 <i>μ</i>M CQ for 2 hrs (b) followed by treatment with 1 <i>μ</i>M TSA or 5 <i>μ</i>M SAHA for 24 hours. Total lysates were then extracted and subjected to Western blot analysis with indicated antibodies.</table>

BioMed Research International

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Figure 4

Figure 4: Trichostatin A Suppresses EGFR Expression through Induction of MicroRNA-7 in an HDAC-Independent Manner in Lapatinib-Treated Cells 