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BioMed Research International
Volume 2014, Article ID 169071, 7 pages
Research Article

Comparison of Extraction Methods of Chitin from Ganoderma lucidum Mushroom Obtained in Submerged Culture

1Biotechnology Group, Natural Sciences Faculty, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Antioquia, Colombia
2Biomaterials and Biomechanical Research Group, Bioengineering Program, Universidad de Antioquia, Calle 67 No. 53-108, Bloque 18-227 Medellín, Colombia

Received 25 April 2013; Accepted 22 October 2013; Published 15 January 2014

Academic Editor: Alberto Reis

Copyright © 2014 Sandra Patricia Ospina Álvarez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The chitin was isolated from the Ganoderma lucidum submerged cultures mycelium as potential source of chitin under biotechnological processes. The extraction of chitin was carried out through 5 different assays which involved mainly three phases: pulverization of the mushroom, deproteinization of the mycelia with NaOH solution, and a process of decolorization with potassium permanganate and oxalic acid. The chitin contents extracted from 9-day mycelia were 413, 339, 87, 78, and 144 mg/g−1 (milligrams of chitin/grams of dry biomass) for A1, A2, A3, A4, and A5, respectively. Obtained chitin was characterized by X-Ray Diffraction (XRD), by Fourier transform infrared spectroscopy (FTIR), and by thermal analysis (TGA). The results showed that Ganoderma lucidum chitin has similar characteristic of chitin from different fonts. The advantage of the biotechnological processes and the fact that Ganoderma lucidum fungus may be used as a potential raw material for chitin production were demonstrated.