p53 Is a Key Regulator for Osthole-Triggered Cancer Pathogenesis
Figure 5
Reactive oxygen species involved in osthole-induced p53 activation. SW480 cells were incubated with H2O2 (200 μM; (a)), H2O2 plus N-acetyl-L-cysteine (H2O2 + NAC; (b)), osthole (10 μM; (c)), or osthole plus N-acetyl-L-cysteine (osthole + NAC; (d)) for 120 min. ROS generations were determined using flow cytometry with the fluorescence probe H2DCFH-DA. (e) Cells were treated with NAC plus osthole for 24 h and the protein expressions of p53, p-p53, and acetyl-p53 were examined by western blot analysis. Results are the representative of three independent experiments.
