Interaction Analysis through Proteomic Phage Display
Figure 2
Schematic representation of the assembly and excretion of the M13 filamentous phages and the exit through cell lysis of the lytic T7 phage. (a) The filamentous M13 phage is assembled at the cell membrane of the bacterial host. In the host cell, the ssDNA is protected by association with protein pV, which detaches at the membrane upon assembly. At the start of the assembly, membrane associated pVII/pIX bind a specific DNA packing signal. Membrane bound protein pVIII binds to the DNA and is transferred across the membranes. The transport is facilitated by a complex of pI and pXI situated in the inner membrane and protein pIV that makes a pore through the outer membrane of the bacteria for the phage to pass through. As a final step, pVI and pIII that span the inner membrane are assembled on the phage. The figure was created based on [31]. (b) The lytic T7 phage, schematically shown with its typical icosahedral head, is assembled in the cytosol of the host cell. It is multiplied to such extent that the host cell finally bursts and the phage is released to the surrounding.
