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BioMed Research International
Volume 2014, Article ID 179836, 8 pages
Research Article

Induction of Apoptotic Effects of Antiproliferative Protein from the Seeds of Borreria hispida on Lung Cancer (A549) and Cervical Cancer (HeLa) Cell Lines

Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, India

Received 25 July 2013; Revised 8 December 2013; Accepted 10 December 2013; Published 29 January 2014

Academic Editor: Stelvio M. Bandiera

Copyright © 2014 S. Rupachandra and D. V. L. Sarada. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure 1: The active protein fraction – F3 at concentrations ranging from 10-1000µg/mL exhibited increased cytotoxic activity in A549 cells, evaluated by MTT assay associated with chromatin condensation in the tested cell line as evidenced in the microscopic observations. Arrow marks indicate the presence of apoptotic cells.

Figure 2: Potent cytotoxic activity was noted in HeLa cells treated with 10-1000µg/mL of protein fraction – F3, analysed by MTT assay, resulted in the fragmentation of cell nucleus in the tested cell line as depicted in the micrographs. Arrow marks indicate the presence of apoptotic cells.

Figure 3: Flow cytometry analysis of four phases of cell cycle (SubG0, G1, S, G2 and M) in A549 cells treated with protein fraction – F3 at a concentration of 1000µg/mL depicted the induction of apoptosis in A549 cells at SubG0-G1 phase as compared to the non apoptotic population in cell control (untreated A549 cells).

Figure 4: HeLa cell cycle analysis (SubG0, G1, S, G2 and M phases) by Flow cytometry revealed the apoptotic effect of protein fraction – F3 at a concentration of 1000µg/mL, showing increased number of apoptotic cells at SubG0-G1 phase in HeLa cells and absence of apoptosis in control (untreated HeLa cells).

  1. Supplementary Material