Research Article
Rapid and Sensitive PCR-Dipstick DNA Chromatography for Multiplex Analysis of the Oral Microbiota
Table 2
Procedure for semiquantification of plaque samples.
| Procedure | Item | Amount (volume) |
| Plaque sampling | Dental plaque | 1.5 mg |
| DNA extraction | InstaGene Matrix Kit | 300 µL |
| PCR | DNA extraction used for PCR | 21 µL | Primer F (tagged) (5 µM) | 2 µL | Primer R (biotinylated) (5 µM) | 2 µL | Hot StarTaq PLUS Master Mix | 25 µL |
| Dipstick DNA chromatography | DNA amplified by PCR | 1 µL | Deionized water | 29 µL | Eluent (supplied by TBA Co.; containing detergents, blocking agents, PBS, and salts solution) | 10 µL | Streptavidin-coated blue latex suspension (supplied by TBA Co.) | 2 µL |
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