Bortezomib Treatment Produces Nocifensive Behavior and Changes in the Expression of TRPV1, CGRP, and Substance P in the Rat DRG, Spinal Cord, and Sciatic Nerve

Table 2

Antibodies used for immunohistochemistry.

Antigen	Immunogen	Manufacturer, species, and type	Dilution used
TRPV1	Synthetic peptide, RASLDSEESESPPQENSC; corresponding to aa 4–21 of rat; TRPV1	Neuromics; Rabbit; Polyclonal	1 : 6000 (ABC), 1 : 1000 (IF),
	Synthetic peptide,; CGSLKPEDAEVFKDSMVPGEK; corresponding to C-terminal residues 816–838 of rat VR1	AbCam; Rabbit; Polyclonal	1 : 600 (ABC), 1 : 100 (IF), 1 : 1000 (WB)
	Epitope mapping near N-terminus of VR1 of rat origin	SantaCruz Biotechnology; Goat; Polyclonal	1 : 800 (ABC), 1 : 100 (IF)
CGRP	Synthetic CGRP from rat	Chemicon Int.; Rabbit; Polyclonal	1 : 1200 (ABC), 1 : 600 (IF)
	Synthetic rat alpha-CGRP conjugated to bovine serum albumin using glutaraldehyde	Enzo Life Sciences; Rabbit; Polyclonal	1 : 5000 (ABC), 1 : 2000 (IF)
	Rat alpha-CGRP peptide	AbCam; Mouse; Monoclonal	1 : 500 (ABC), 1 : 100 (IF)
SP	Synthetic peptide: CRPKPQQFFGLM, corresponding to amino acids 1–11 of rat substance P	AbCam; Guinea pig; Polyclonal	1 : 1500 (ABC), 1 : 500 (IF)
GAPDH	GAPDH from rabbit muscle	Millipore Chemicon	1 : 1000 (WB)