Glioprotective Effects of Ashwagandha Leaf Extract against Lead Induced Toxicity
Expression of GFAP after treatment with ASH-WEX and lead nitrate. Control (C), control with ASH-WEX (0.1%) pretreatment (CA), lead nitrate 200 μM (LN), and lead nitrate after pretreatment with ASH-WEX (LN + AS) cells were used for GFAP immunostaining (a) protein expression by Western blotting (c) and mRNA expression by RT-PCR (d). (b) depicts GFAP expression levels as analysed by immunostaining using intensity measurement command of Image-Pro Plus software. GFAP protein expression levels in control cells were taken as 100% to plot the histogram with relative level of expression of GFAP in C, CA, LN, and LN + AS groups. Data are calculated from three independent experiments and represented as the mean ± SEM. Value of was considered to be significant. Significant difference as compared to control, #significant difference between LN and LN + AS groups. Significant difference between C and other groups and #significant difference between LN and LN + AS groups.
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