Glioprotective Effects of Ashwagandha Leaf Extract against Lead Induced Toxicity

Table 1

Primer sequences used for semiquantitative RT-PCR.

Number	mRNA		Primer sequence	Expected product size
1	GFAP	F	5′GGC GCT CAA TGC TGG CTT CA3′	326 bp
		R	5′TCT GCC TCC AGC CTC AGG TT3′
2	HSP70	F	5′GAG TTC AAG CGC AAA CAC AA3′	428 bp
		R	5′CTC AGA CTT GTC GCC AAT GA3′
3	NCAM	F	5′GCC AAG GAG AAA TCA GCG TTG GAG AGT C3′	651 bp
		R	5′ATG CTC TTC AGG GTC AAG GAG GAC ACA C3′
4	Mortalin	F	5′CAG TCT TCT GGT GGA TTA AG3′	420 bp
		R	5′ATT AGC ACC GTC ACG TAA CAC CTC3′
5	𝛽-Actin	F	5′TCACCCACACTGTGCCCATCTACGA3′	285 bp
		R	5′CAGCGGAACCGCTCATTGCCAATGG3′