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BioMed Research International
Volume 2014 (2014), Article ID 185946, 7 pages
Research Article

Evaluation of the Antioxidant Activity and Antiproliferative Effect of the Jaboticaba (Myrciaria cauliflora) Seed Extracts in Oral Carcinoma Cells

1Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan
2Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan
3School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan
4Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan
5National Sun Yat-Sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 804, Taiwan
6Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
7Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
8Institute of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan 717, Taiwan
9Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 300, Taiwan
10Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
11Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
12School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
13Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
14Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan

Received 29 March 2014; Revised 6 July 2014; Accepted 22 July 2014; Published 18 August 2014

Academic Editor: Blanca Hernández-Ledesma

Copyright © 2014 Wen-Hung Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the antiproliferative activity of extracts from different parts of the jaboticaba (Myrciaria cauliflora) plant was evaluated for its effect on human oral carcinoma cell lines. The cytotoxicities of various plant extract concentrations were examined and the 50% maximal inhibitory concentration (IC50) was determined. Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects. Annexin V/propidium iodide positivity with active caspase-3 induction indicated that the treated cells underwent apoptosis. Several important regulatory proteins (Bcl-2, Bcl-xL, Bid, and survivin) involved in apoptosis were also evaluated. The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined. The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.