Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells
Figure 2
Identification of neural crest-derived DPCs. ((a)–(c)) Immunofluorescent cytochemical staining revealed expression of NCSCs-specific markers in DPCs. Primary DPCs were double positive for P75 and Sox10 (a), P75 and Nestin (b), and were positive for Sox9 (c). (d) RT-PCR analysis confirmed the expression of dermal papillae markers ALP and Sox2, and the NCSCs markers Nestin, P75, Sox9, Twist1, and AP2 in DPCs, as well as BMSCs. Scale bar = 20 μm ((a)–(c)). DPCs-P#: DPCs at passage #; BMSCs-P#: BMSCs at passage #.
