Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells
Figure 3
Mesenchymal stem cell phenotype and multipotential differentiation assay of DPCs. ((a)–(d)) DPCs exhibited a mesenchymal stem cell phenotype. Immunofluorescent staining demonstrated that DPCs expressed CD44 (a) and CD90 (b), but did not express CD31 (c). Flow cytometry analysis further demonstrated a high positive expression rate of CD90 and CD105, as well as a low positive expression rate of CD31 (d). ((e)–(h)) Multipotential differentiation capacity of DPCs. (e) Adipogenic differentiation of DPCs. The intracellular lipid droplets were formed after induction for 2 weeks and were detected by Oil-Red O staining. (f) Osteogenic differentiation of DPCs. Calcium nodules were formed after induction for 2 weeks and detected by Alizarin Red-S staining. (g) SMC differentiation of DPCs. A proportion of the DPCs were positive for -SMA after induction for 1 week. (h) Neuronal differentiation of DPCs. A proportion of DPCs (white arrow) exhibited a neuron-like morphology and were positive for neuron-specific 3-Tubulin after induction for 1 week. Scale bar = 50 μm ((a)–(c), (f)) and 20 μm ((e), (g), (h)).
