Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells

Figure 5

DPCs promoted neurite outgrowth in PC12 cells and secreted BDNF and GDNF. ((a)–(h)) After culturing for 4 days, the average neurite lengths of PC12 cells cultured in DPC-CM supplemented with various concentrations of NGF ((a)–(d)) were longer than those of cells cultured in BM supplemented with the same concentrations of NGF ((e)–(h)), as indicated by phase contrast microscopic observation. (i) The PC12 cells cultured in DPC-CM supplemented with 10 ng/mL NGF displayed the longest average neurite lengths compared with other concentrations of NGF (0 ng/mL, 5 ng/mL, and 30 ng/mL), but there was no statistical difference with high dose NGF (30 ng/mL) (𝑃>0.05). ((j), (k)) The amount of BDNF (j) and GDNF (k) secreted per 10 thousand cells in 5 mL DPC-CM, BMSC-CM, and BM for 24 h. Scale bar = 20 μm ((a)–(h)). ^∗𝑃<0.05. ^∗∗𝑃<0.01.

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