Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells

Table 1

List of all primers used with PCR and amplification conditions.

Gene	Primer sequence	Annealing temperature
ALP	TCCATGGTGGATTATGCTCA; TTCTGTTCCTGCTCGAGGTT	60°C
Nestin	ACTGCAGAAGAGGACCTGGA; AGTTCCCACTCCTGTGGTTG	56°C
Sox2	GCACATGAACGGCTGGAGCAACG; TGCTGCGAGTAGGACATGCTGTAGG	65°C
P75	GGCTACTACCAGGACGAGGAG; GCCAAGATGGAGCAATAGACA	58°C
Sox9	GTGCTGAAGGGCTACGACTGGA; GTTGTGCAGATGCGGGTACTGG	64°C
Twist1	CATCCTTGTGGACTTTCTCCA; GCATTTTACCATGGGTCATCA	60°C
AP2α	TGGGCACTGTAGGTCAATCTC; GTTAATAGGGATGGCGGAGAC	60°C
BDNF	TGGCTGACACTTTTGAGCAC; GCAGCCTTCCTTCGTGTAAC	56°C
GDNF	GACTCCAATATGCCCGAAGA; ATGGTAAACCAGGCTGTCGT	56°C
NGF	GGACGCAGCTTTCTATCCTG; GTCCGTGGCTGTGGTCTTAT	56°C
GAPDH	ATGGGAAGCTGGTCATCAAC; GGATGCAGGGATGATGTTCT	58°C