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BioMed Research International
Volume 2014, Article ID 187105, 11 pages
Research Article

Genome-Wide Uncovering of STAT3-Mediated miRNA Expression Profiles in Colorectal Cancer Cell Lines

1School of Life Science and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China
2College of Engineering, South China Agricultural University, Guangzhou 510642, China
3Experimental Research Center, First People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China
4Biomedical Research Institute, Shenzhen PKU-HKUST Medical Center, No. 1120, Lianhua Road, Futian District, Shenzhen 518036, China

Received 31 December 2013; Accepted 19 June 2014; Published 13 July 2014

Academic Editor: Zhifan Jia

Copyright © 2014 Jufeng Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1: Sequencing data of SW480/STAT3-siRNA and SW480/siRNA-control.

Table S2: Gene Ontology enrichment analysis of predicted targets of differentially expressed novel miRNAs.

Table S3: The KEGG analysis of predicted targets mediated by novel miRNAs.

Figure S1: Size distributions of small RNAs in SW480/siRNA-control and SW480/STAT3-siRNA cells. 22-nucleotide small RNAs are enriched in both samples, which is in line with the typical size of miRNAs.

Figure S2: Base compositions of small RNAs in SW480/siRNA-control and SW480/STAT3-siRNA cells. Each color represents the small RNA tags whose first base is a certain base. Most 22-nt small RNAs begin with the base “U”. The 23-nt small RNAs display a bias to “A” and “U” at first base in SW480/STAT3-siRNA while only “U” in SW480/siRNA-control.

Figure S3: The proportions of unique common tags and total common tags of small RNAs detected in SW480/siRNA-control and SW480/STAT3-siRNA cells. The very few unique common tags indicate that SW480/STAT3-siRNA presents a distinctive small RNA profile compared to SW480/siRNA-control.

Figure S4: Mapping of small RNAs from SW480/siRNA-control and SW480/STAT3-siRNA cells onto human chromosomes, respectively. Red lines indicate miRNAs that are located on the antisense chains of genome while the blue ones are on the sense chains.

  1. Supplementary Material