Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells
Figure 2
Enzymatic activity and myotoxic activity of PLA2s isolated from the venom of B. mattogrossensis. (a) Phospholipase activity of the fractions collected from the rechromatography of fraction 9 done in C18 column assayed using an NOB stained substrate. This activity was assessed through the measurement of the number of moles of the released chromophore per minute (n°mols/min or U) per milligram of protein. (b) Phospholipase activity of the fractions collected from the rechromatography of fraction 9 done in C18 column assayed using a fluorescent substrate. (c) Myotoxic activity evaluated for inoculation of PLA2s (25 μg/50 µL) or PBS (control) done intramuscularly, in the gastrocnemius muscle of mice. After 3 hours, the creatine kinase (CK) level, an important marker of muscular lesion, was assayed in the animal’s plasma. Each bar represents the average +/− SD of three independent groups. * compared to the control. F13: BmatTX-I (Lys49); F14: BmatTX-II (Lys49); F15: BmatTX-III (Asp49); Controls: BthTX-I (Lys49) and BthTX-II (Asp49).
