Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells
Enzymatic activity and myotoxic activity of PLA2s isolated from the venom of B. mattogrossensis. (a) Phospholipase activity of the fractions collected from the rechromatography of fraction 9 done in C18 column assayed using an NOB stained substrate. This activity was assessed through the measurement of the number of moles of the released chromophore per minute (n°mols/min or U) per milligram of protein. (b) Phospholipase activity of the fractions collected from the rechromatography of fraction 9 done in C18 column assayed using a fluorescent substrate. (c) Myotoxic activity evaluated for inoculation of PLA2s (25 μg/50 µL) or PBS (control) done intramuscularly, in the gastrocnemius muscle of mice. After 3 hours, the creatine kinase (CK) level, an important marker of muscular lesion, was assayed in the animal’s plasma. Each bar represents the average +/− SD of three independent groups. * compared to the control. F13: BmatTX-I (Lys49); F14: BmatTX-II (Lys49); F15: BmatTX-III (Asp49); Controls: BthTX-I (Lys49) and BthTX-II (Asp49).