BioMed Research International / 2014 / Article / Fig 7

Research Article

Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells

Figure 7

Antileishmanial activity of the crude venom of and PLA2s from B. mattogrossensis. The promastigote forms of L. amazonensis were plated with  cells/well. Then, different concentrations of crude venom and isolated PLA2s were added to each well. The experiment was done in a 48 h period, with the (a) crude venom and the PLA2 enzymes, (b) BmatTX-I, and (c) BmatTX-III. MTT was added and after the incubation period at 33°C, the formazan crystal formed was dissolved in SDS. Readings were done in a spectrophotometer at 570 nm. Each bar represents the average +/− SD of the three independent experimental groups, sixfold total. * compared to the control.