Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells
Antileishmanial activity of the crude venom of and PLA2s from B. mattogrossensis. The promastigote forms of L. amazonensis were plated with cells/well. Then, different concentrations of crude venom and isolated PLA2s were added to each well. The experiment was done in a 48 h period, with the (a) crude venom and the PLA2 enzymes, (b) BmatTX-I, and (c) BmatTX-III. MTT was added and after the incubation period at 33°C, the formazan crystal formed was dissolved in SDS. Readings were done in a spectrophotometer at 570 nm. Each bar represents the average +/− SD of the three independent experimental groups, sixfold total. * compared to the control.