In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells

Figure 4

Agarose gel electrophoresis of HCV nested PCR products in two different types of cells treated with S-ODN1 and its derivative. HCV infected HepG2 cells and PBMC were treated with S-ODN1 for 24 and 48 hours. Lanes HC (+, −): untreated HCV infected HepG2 cells cultured for 48 h amplified plus and minus strands, respectively, and lanes H 24 (+, −): plus and minus strands PCR products, respectively, for HCV infected HepG2 cells treated with S-ODN1 for 24 h, lanes HS 24 (+, −): plus and minus strands PCR products, respectively, for HCV infected HepG2 cells treated with S-ODN1 derivative for 24 h, lanes H 48 (+,−): plus and minus strands PCR products, respectively, for HCV infected HepG2 cells treated with S-ODN1 for 48 h, lanes HS 48 (+, −): plus and minus strands PCR products, respectively, for HCV infected HepG2 cells treated with S-ODN1 derivative for 48 h, lanes PC (+, −): untreated HCV infected PBMC cultured for 48 h amplified plus and minus strands, respectively, +ve: positive PCR control, and lanes P 24 (+, −): plus and minus strands PCR products, respectively, for HCV infected PBMC treated with S-ODN1 for 24 h, lanes PS 24 (+, −): plus and minus strands PCR products, respectively, for HCV infected PBMC treated with S-ODN1 derivative for 24 h, lanes P 48 (+, −): plus and minus strands PCR products, respectively, for HCV infected PBMC treated with S-ODN1 for 48 h, lanes PS 48 (+, −): plus and minus strands PCR products, respectively, for HCV infected PBMC treated with S-ODN1 derivative for 48 h, –ve: is negative PCR control.