Research Article
Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue
Table 1
Stability of genes candidates to normalize RT-qPCR for thyroid derived biological material.
| Study | Material | Reference gene stability | Method |
|
Santin et al., 2013 [9] | Human normal cells | ACTB>TBP>GAPDH>B2M | NormFinder | Chantawibul et al., 2012 [10] | Human tissues* | GAPDH>ACTB>B2M>RPLIA>HPRT1 | NormFinder |
Lecchi et al., 2012 [11] | Bovine tissues, including thyroid | SF3A1=HBMS>ACTB | geNorm | HMBS>ACTB>GAPDH | NormFinder |
Li et al., 2011 [12] | Porcine thyroid tissue | 18S rRNA>Ubiquitin>Histone H3>ACTB | geNorm | 18S rRNA>Ubiquitin>Histone H3>ACTB>GAPDH | NormFinder | Lisowski et al., 2008 [13] | Bovine thyroid tissue | TBP=HPRT1>YWHAZ>ACTB | geNorm |
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13 goiters, 6 adenomas, 4 carcinomas (2 follicular + 2 papillary), and 2 lymphocytic thyroiditis.
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