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BioMed Research International
Volume 2014, Article ID 198697, 5 pages
Research Article

IDH1R132H Mutation Increases U87 Glioma Cell Sensitivity to Radiation Therapy in Hypoxia

1Université Pierre et Marie Curie-Paris 6, Centre de Recherche de l’Institut du Cerveau et de la Moëlle épinière (CRICM), UMR-S975, 75013 Paris, France
2INSERM, U 975, 75013 Paris, France
3CNRS, UMR 7225, 75013 Paris, France
4CNRS, UMR 6301 ISTCT, CERVOxy group, GIP CYCERON, Boulevard Henri Becquerel, BP 5229, 14074 Caen cedex, France
5Université de Caen Basse-Normandie, UMR 6301 ISTCT, 14000 Caen, France
6CEA, DSV/I2BM, UMR 6301 ISTCT, 14000 Caen, France
7CHU de Caen, Service de Neurologie, Boulevard Côte de Nacre, 14000 Caen, France
8AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service de Neurologie 2, 75013 Paris, France
9Service de Neurologie 2, Groupe Hospitalier Pitié-Salpêtrière, 75651 Paris Cedex 13, France

Received 12 February 2014; Accepted 6 April 2014; Published 7 May 2014

Academic Editor: Andrea Pace

Copyright © 2014 Xiao-Wei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. IDH1 codon 132 mutation (mostly Arg132His) is frequently found in gliomas and is associated with longer survival. However, it is still unclear whether IDH1 mutation renders the cell more vulnerable to current treatment, radio- and chemotherapy. Materials and Methods. We transduced U87 with wild type IDH1 or expressing lentivirus and analyzed the radiosensitivity (dose ranging 0 to 10 Gy) under normoxia (20% O2) and moderate hypoxia (1% O2). Results. We observed that U87 cells grow faster in hypoxia and were more sensitive to radiotherapy (in terms of cell mortality and colony formation assay) compared to nontransduced U87 and IDH1wt cells. This effect was not observed in normoxia. Conclusion. These data suggest that mutation increases radiosensitivity in mild hypoxic conditions.