IDH1R132H Mutation Increases U87 Glioma Cell Sensitivity to Radiation Therapy in Hypoxia
Figure 5
IDH1 R132H-U87 cells have a reduced colony forming cell ability after irradiation in hypoxia. U87, IDH1 wt-U87, and IDH1 R132H-U87 cells were plated 24 h before irradiation (0-2-4-6-8 Gy) in agar and incubated for one month in normoxia (20% O2) and in hypoxia (1% O2). The colonies were fixed in ethanol, stained with 0.05% crystal violet, and counted. Survival rate was estimated by the ratio between the colonies count and the number of cells plated, multiplicated by the plating efficiency.
