IL-6 Impairs Myogenic Differentiation by Downmodulation of p90RSK/eEF2 and mTOR/p70S6K Axes, without Affecting AKT Activity
Figure 1
Determination of secreted cytokines in the conditioned medium from C-26 adenocarcinoma cells. (a–f) MCP-1, IL-6, IFN-γ, IL-10, TNF-α, and IL-12p70 protein levels were measured using a cytometric bead assay in which the specific antibodies were conjugated with capture beads (see Section 2). MCP-1 concentration is expressed in ng/mL, whereas the other cytokines concentrations are in pg/mL. 1: control I, conditioned medium (CM) from proliferating C2C12 cells in growth medium (GM); 2: control II, myogenic differentiation medium (DM); 3: CM from proliferating C-26 cells in GM (i.e., exponential phase, with a cell confluence about 50%); 4, 5, and 6: CM from confluent C-26 cells (i.e., stationary phase) with a cell confluence of about 100% for 1, 2, or 3 days, respectively. The experiment indicates means ± standard deviation from three independent determinations.
