Potent Protein Glycation Inhibition of Plantagoside in Plantago major Seeds
Figure 2
Inhibition of cross-ink formation by plantagoside (1). This assay was performed at pH 7.4 in phosphate buffer, which contained 20 mM ribose, 5 mg/mL lysozyme, 100 mM of aminoguanidine or NaCNBH3, or the indicated concentration of plantagoside dissolved in dimethylsulfoxide, for 1 week at 37°C. Each sample was then subjected to 17.5% SDS-PAGE and stained with Coomassie Brilliant Blue. DMSO: dimethylsulfoxide containing no inhibitor; AG: aminoguanidine.
