The Soluble Form of CTLA-4 from Serum of Patients with Autoimmune Diseases Regulates T-Cell Responses
sCTLA-4 from autoimmune sera is able to decrease the secretion of activator cytokines. Soluble CTLA-4 containing sera from autoimmune patients are able to decrease the production of interferon-γ (IFN-γ), interleukin-2 (IL-2), IL-13, and IL-7. These data are confirmed by control experiment with sera of autoimmune patients that were negative for sCTLA-4 through ELISA test. Positive or negative sera are indicated by an alphanumeric code followed by and , respectively. CTLA-4 Ig fusion protein is a positive control of secretion of cytokines. The concentration of activator cytokines is expressed in pg/mL. The data represent the averages standard deviations (error bars) of double samples. A representative experiment out of three is shown.