A Mushroom Extract Piwep from Phellinus igniarius Ameliorates Experimental Autoimmune Encephalomyelitis by Inhibiting Immune Cell Infiltration in the Spinal Cord
Figure 4
Piwep treatment attenuates mRNA expression of surface molecules in the spinal cord of EAE mice. Spinal cord perfused with cold PBS was used for extraction of total RNA. Expression of mRNA was determined with the RT-PCR method. RT-PCR results demonstrate that T cell, B cell, and microglia/macrophage labeled cells extensively infiltrated the white matter of EAE mice (MOG + vehicle). However, Piwep significantly reduced the EAE-associated increase in mRNA expression of CD11b, CD4, CD8, and CD20. Graphs represent quantified CD11b, CD4, CD8, and CD20 expression with or without Piwep treatment in EAE mice. Data are mean SEM (). compared with Piwep treated group.
