Circulating Conventional and Plasmacytoid Dendritic Cell Subsets Display Distinct Kinetics during In Vivo Repeated Allergen Skin Challenges in Atopic Subjects
Figure 7
Activin-A modifies the chemokine receptor profile of CD1c+ cDCs towards a skin-homing phenotype. CD1c+ cDCs were isolated from the peripheral blood of individuals with atopy to Dermatophagoides pteronyssinus (Der p1), cultured for 24 h with 1 μg/mL Der p1 in the presence of 50 ng/mL recombinant activin-A or PBS (control). DCs were stained with fluorochrome-conjugated antibodies against human CCR4, CCR10, CCR6, CCR9, and CXCR3 and analysed by flow-cytometry. (a) Activin-A induced an increase in CCR10 levels, concomitant with a decrease in CCR4 on CD1c+ cDCs during stimulation with Der p1 in vitro. No differences were observed regarding the expression of CCR6, CCR9, and CXCR3 in Der p1-stimulated CD1c+ cDCs in the presence or absence of activin-A. (b) Activin-A significantly increased the ratio of CCR10/CCR4 expressing cDCs. Data are representative of two independent experiments. *.
