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BioMed Research International
Volume 2014, Article ID 231589, 14 pages
http://dx.doi.org/10.1155/2014/231589
Research Article

Retrotransposon-Based Molecular Markers for Analysis of Genetic Diversity within the Genus Linum

1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia
2Department of Higher Plants, Lomonosov Moscow State University, Moscow 119991, Russia
3Research Institute of Physico-Chemical Medicine, Moscow 119435, Russia
4Institute of Genetics and Cytology, National Academy of Science of Belarus, 220072 Minsk, Belarus
5Research Center for Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia
6All-Russian Research Institute for Flax of the Russian Academy of Agricultural Sciences, Torzhok 172002, Russia

Received 25 April 2014; Revised 18 July 2014; Accepted 1 August 2014; Published 27 August 2014

Academic Editor: Peter F. Stadler

Copyright © 2014 Nataliya V. Melnikova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

SSAP method was used to study the genetic diversity of 22 Linum species from sections Linum, Adenolinum, Dasylinum, Stellerolinum, and 46 flax cultivars. All the studied flax varieties were distinguished using SSAP for retrotransposons FL9 and FL11. Thus, the validity of SSAP method was demonstrated for flax marking, identification of accessions in genebank collections, and control during propagation of flax varieties. Polymorphism of Fl1a, Fl1b, and Cassandra insertions were very low in flax varieties, but these retrotransposons were successfully used for the investigation of Linum species. Species clusterization based on SSAP markers was in concordance with their taxonomic division into sections Dasylinum, Stellerolinum, Adenolinum, and Linum. All species of sect. Adenolinum clustered apart from species of sect. Linum. The data confirmed the accuracy of the separation in these sections. Members of section Linum are not as closely related as members of other sections, so taxonomic revision of this section is desirable. L. usitatissimum accessions genetically distant from modern flax cultivars were revealed in our work. These accessions are of utmost interest for flax breeding and introduction of new useful traits into flax cultivars. The chromosome localization of Cassandra retrotransposon in Linum species was determined.