Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia

Table 4

Distribution in Ethiopia and neighboring subregions of 11 predominant spoligotype patterns in this study (clusters containing 3 or more isolates), as seen through the updated SITVIT2 database^a.

Octal code (SIT) lineage

Ethiopia

𝑃 value

Distribution in five African subregions and neighboring Western Asia in SITVIT2^b

This study

SITVIT2

AFRI-E

𝑃-value

AFRI-N

𝑃 value

AFRI-W

𝑃 value

AFRI-M

𝑃 value

AFRI-S

𝑃 value

ASIA-W

𝑃 value

𝑁/𝑡 (%)

𝑁/𝑡 (%)

𝑁/𝑡 (%)

𝑁/𝑡 (%)

𝑁/𝑡 (%)

𝑁/𝑡 (%)

𝑁/𝑡 (%)

𝑁/𝑡 (%)

37 (T3)

10/105

77/1507

0.072

90/4138

0.0003*

7/2145

<0.0001*

6/1985

<0.0001*

0/808

<0.0001*

17/4580

<0.0001*

55/4790

<0.0001*

777737777760771

(9.52)

(5.11)

(2.17)

(0.33)

(0.30)

(0.00)

(0.37)

(1.15)

149 (T3-ETH)

10/105

271/1507

0.056

271/4138

0.26

1/2145

<0.0001*

0/1985

<0.0001*

0/808

<0.0001*

1/4580

<0.0001*

34/4790

<0.0001*

777000377760771

(9.52)

(17.98)

(6.55)

(0.05)

(0.00)

(0.00)

(0.02)

(0.71)

53 (T1)

9/105

154/1507

0.62

283/4138

0.52

221/2145

0.60

109/1985

0.21

24/808

0.006*

312/4580

0.51

459/4790

0.75

777777777760771

(8.57)

(10.22)

(6.84)

(10.30)

(5.49)

(2.97)

(6.81)

(9.58)

54 (Manu2)

9/105

0/1507

<0.0001*

4/4138

<0.0001*

36/2145

<0.0001*

3/1985

<0.0001*

0/808

<0.0001*

16/4580

<0.0001*

42/4790

<0.0001*

777777777763771

(8.57)

(0.00)

(0.10)

(1.68)

(0.15)

(0.00)

(0.35)

(0.88)

52 (T2)

6/105

24/1507

0.0036*

40/4138

0.001*

6/2145

<0.0001*

6/1985

<0.0001*

33/808

0.44

21/4580

<0.0001*

49/4790

0.0013*

777777777760731

(5.71)

(1.59)

(0.97)

(0.28)

(0.30)

(4.08)

(0.46)

(1.02)

25 (CAS1-Delhi)

4/105

144/1507

0.066

151/4138

0.79

84/2145

1.0

2/1985

<0.0001*

1/808

0.0009*

3/4580

<0.0001*

168/4790

0.79

703777740003171

(3.81)

(9.56)

(3.65)

(3.92)

(0.10)

(0.12)

(0.07)

(3.51)

26 (CAS1-Delhi)

4/105

43/1507

0.55

71/4138

0.12

16/2145

0.013*

0/1985

<0.0001*

0/808

0.0002*

6/4580

<0.0001*

213/4790

1.0

703777740003771

(3.81)

(2.85)

(1.72)

(0.75)

(0.00)

(0.00)

(0.13)

(4.45)

119 (X1)

3/105

1/1507

0.001*

4/4138

0.0005*

5/2145

0.005*

2/1985

0.0012*

0/808

0.0016*

143/4580

1.0

9/4790

0.002*

777776777760771

(2.86)

(0.07)

(0.10)

(0.23)

(0.10)

(0.00)

(3.12)

(0.19)

121 (H3)

3/105

7/1507

0.024*

7/4138

0.002*

0/2145

0.0001*

0/1985

0.0001*

0/808

0.0016*

0/4580

<0.0001*

3/4790

0.0002*

777777775720771

(2.86)

(0.46)

(0.17)

(0.00)

(0.00)

(0.00)

(0.00)

(0.06)

523 (Manu_ancestor)

3/105

0/1507

0.0003*

0/4138

<0.0001*

1/2145

0.0004*

4/1985

0.004*

0/808

0.0016*

2/4580

0.0001*

4/4790

0.0003*

777777777777771

(2.86)

(0.00)

(0.00)

(0.05)

(0.20)

(0.00)

(0.04)

(0.08)

3897 (Manu2)

3/105

0/1507

0.0003*

0/4138

<0.0001*

0/2145

0.0001*

0/1985

0.0001*

0/808

0.0016*

0/4580

<0.0001*

0/4790

<0.0001*

777347777763671

(2.86)

(0.00)

(0.00)

(0.00)

(0.00)

(0.00)

(0.00)

(0.00)

aAsterisk denotes statistically significant differences (𝑃<0.05) as compared to the total number of strains for a given pattern in this study (𝑛=105 strains). For statistical comparison of differences observed, note that Pearson’s Chi-square test was used when more than 80% of data had an expected value greater than 5, and Fisher’s Exact Test for remaining data with smaller values (at least 20% of data having values less than 5).
^bThe definition of subregions is according to the United Nations (http://unstats.un.org/unsd/methods/m49/m49regin.htm). The table summarizes distribution in this study (𝑛=105) versus all strains reported from Ethiopia in the international database (𝑛=1507, excluding the present study), the five African subregions (𝑛=13656), and neighboring Western Asian countries (ASIA-W, 𝑛=4790). AFRI-E: Burundi, Comoros, Ethiopia, Kenya, Madagascar, Malawi, Mauritius, Mozambique, Reunion, Tanzania, Uganda, Zambia, and Zimbabwe; AFRI-N: Algeria, Egypt, Libyan Arab Jamahiriya, Morocco, Sudan, and Tunisia; AFRI-W: Benin, Burkina Faso, Gambia, Guinea, Guinea-Bissau, Ivory Coast, Mali, Mauritania, Nigeria, Senegal, and Sierra Leone; AFRI-M: Cameroon, Central African Republic, Chad, and Democratic Republic of Congo; AFRI-S: Botswana, Namibia, South Africa, and Swaziland; ASIA-W: Armenia, Azerbaijan, Georgia, Iraq, Israel and Palestinian territories, Oman, Saudi Arabia, Turkey, and Yemen (SITVIT2 database comparison made on August 21st 2013 by David Couvin and Nalin Rastogi).