Seroprevalence of Antibodies against Pkn1, a Novel Potential Immunogen, in Chlamydia trachomatis-Infected Macaca nemestrina and Human Patients

Table 2

ELISA results for the seroprevalence of anti-Pkn1, anti-OmpA and anti-LigA antibodies in sera of M. nemestrina (Macaque model) infected with C. trachomatis and control group.

Number of sample	Immune response as; determined by ELISA			Chlamydial infection*
	Pkn1	OmpA	LigA
18	+	+	−	+
10	−	+	−	+
6	+	−	−	+
2	−	−	−	+
10	−	−	−	−

Diluted sera of M. nemestrina were incubated with respective antigens followed by HRP conjugated secondary antibodies using standard protocol as described under methods. 𝐴₄₅₀ above the cut off value was considered as positive samples; the cut-off value for OmpA = 0.40 while cut-off value for Pkn1and LigA was 0.43 and 0.39 respectively. *The status of chlamydial infection was as provided by the laboratory of Prof. Dorothy L. Patton, University of Washington, Seattle, WA, U.S.A.