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BioMed Research International
Volume 2014, Article ID 250408, 6 pages
http://dx.doi.org/10.1155/2014/250408
Research Article

Misidentification of Candida guilliermondii as C. famata among Strains Isolated from Blood Cultures by the VITEK 2 System

1Department of Laboratory Medicine, Inje University College of Medicine, Busan 614-735, Republic of Korea
2Paik Institute for Clinical Research, Inje University College of Medicine, Busan 614-735, Republic of Korea
3Department of Internal Medicine, Pusan National University School of Medicine, Busan 602-739, Republic of Korea
4Biomedical Research Institute, Pusan National University School of Medicine, Busan 602-739, Republic of Korea
5Department of Laboratory Medicine, Pusan National University School of Medicine, Busan 602-739, Republic of Korea
6Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea
7Korean Collection for Type Cultures, Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea
8Department of Dental Hygiene, College of Medical and Life Science, Silla University, Busan 617-736, Republic of Korea
9Department of Internal Medicine, Pusan National University Hospital, 179, Gudeok-ro, Seo-gu, Busan 602-739, Republic of Korea

Received 28 February 2014; Revised 21 April 2014; Accepted 21 April 2014; Published 29 May 2014

Academic Editor: Mina Hur

Copyright © 2014 Si Hyun Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. The aim of this study was to differentiate between Candida famata and Candida guilliermondii correctly by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and gene sequencing. Methods. Twenty-eight Candida strains from blood cultures that had been identified as C. famata (), C. famata/C. guilliermondii (), and C. guilliermondii () by the VITEK 2 system using the YST ID card were included. We identified these strains by MALDI-TOF MS and gene sequencing using the 28S rRNA and ITS genes and compared the results with those obtained by the VITEK 2 system. Results. All 28 isolates were finally identified as C. guilliermondii. Sequencing analysis of the 28S rRNA gene showed 99.80%–100% similarity with C. guilliermondii for all 28 strains. The ITS gene sequencing of the strains showed 98.34%–100% homology with C. guilliermondii. By MALDI-TOF, we could correctly identify 21 (75%) of 28 C. guilliermondii isolates. Conclusion. We should suspect misidentification when C. famata is reported by the VITEK 2 system, and we always should keep in mind the possibility of misidentification of any organism when an uncommon species is reported.