Pu-Erh Tea Extract Induces the Degradation of FET Family Proteins Involved in the Pathogenesis of Amyotrophic Lateral Sclerosis
Figure 1
Purification and identification of the PTE associated protein. (a) Proteins derived from MOLT-3 cells were, respectively, loaded onto the four types of Sepharose 6B beads, and the proteins that were bound to the beads were analyzed on SDS-PAGE gels and stained with Coomassie brilliant blue. (b) A four-step purification procedure of PTE-associated proteins was performed. MOLT-3 cell lysates were sequentially loaded onto Sepharose 6B beads, GTE Sepharose 6B beads, and BTE Sepharose 6B beads, followed by PTE Sepharose 6B beads. The PTE-associated proteins were finally purified with PTE Sepharose 6B beads. The proteins, as indicated by the arrow, were removed from the gel and analyzed using MALDI-TOF MS. (c) SK-N-SH cell lysates and (d) MOLT-3 cell lysates were incubated with PTE Sepharose 6B or Sepharose 6B beads and analyzed by Western blot.
