Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 259238, 8 pages
http://dx.doi.org/10.1155/2014/259238
Research Article

Purification and Characterization of Alkaline-Thermostable Protease Enzyme from Pitaya (Hylocereus polyrhizus) Waste: A Potential Low Cost of the Enzyme

Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia

Received 14 April 2014; Revised 22 July 2014; Accepted 5 September 2014; Published 18 September 2014

Academic Editor: Jose Teixeira

Copyright © 2014 Mehrnoush Amid et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. V. K. Singh, A. K. Patel, A. J. Moir, and M. V. Jagannadham, “Indicain, a dimeric serine protease from Morus indica cv. K2,” Phytochemistry, vol. 69, no. 11, pp. 2110–2119, 2008. View at Publisher · View at Google Scholar · View at Scopus
  2. A. Schaller, “A cut above the rest: the regulatory function of plant proteases,” Planta, vol. 220, no. 2, pp. 183–197, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. C. M. Antão and F. X. Malcata, “Plant serine proteases: biochemical, physiological and molecular features,” Plant Physiology and Biochemistry, vol. 43, no. 7, pp. 637–650, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. R. Tomar, R. Kumar, and M. V. Jagannadham, “A stable serine protease, Wrightin, from the latex of the plant Wrightia tinctoria (Roxb.) R. Br.: purification and biochemical properties,” Journal of Agricultural and Food Chemistry, vol. 56, no. 4, pp. 1479–1487, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. T. Popovič, V. Puizdar, and J. Brzin, “A novel subtilase from common bean leaves,” FEBS Letters, vol. 530, no. 1–3, pp. 163–168, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. H. K. Lim, C. P. Tan, R. Karim, A. A. Ariffin, and J. Bakar, “Chemical composition and DSC thermal properties of two species of Hylocereus cacti seed oil: Hylocereus undatus and Hylocereus polyrhizus,” Food Chemistry, vol. 119, no. 4, pp. 1326–1331, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. C. M. Ajila, S. G. Bhat, and U. J. S. P. Rao, “Valuable components of raw and ripe peels from two Indian mango varieties,” Food Chemistry, vol. 102, no. 4, pp. 1006–1011, 2007. View at Publisher · View at Google Scholar · View at Scopus
  8. L. M. Zanphorlin, H. Cabral, E. Arantes et al., “Purification and characterization of a new alkaline serine protease from the thermophilic fungus Myceliophthora sp,” Process Biochemistry, vol. 46, no. 11, pp. 2137–2143, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Publisher · View at Google Scholar · View at Scopus
  10. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  11. E. Mortz, T. N. Krogh, H. Vorum, and A. Görg, “Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis,” Proteomics, vol. 1, no. 11, pp. 1359–1363, 2001. View at Publisher · View at Google Scholar · View at Scopus
  12. T. Maeda, T. Yoshimura, R. García-Contreras, and H. I. Ogawa, “Purification and characterization of a serine protease secreted by Brevibacillus sp. KH3 for reducing waste activated sludge and biofilm formation,” Bioresource Technology, vol. 102, no. 22, pp. 10650–10656, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. W. Zhu, D. Cha, G. Cheng, Q. Peng, and P. Shen, “Purification and characterization of a thermostable protease from a newly isolated Geobacillus sp. YMTC 1049,” Enzyme and Microbial Technology, vol. 40, no. 6, pp. 1592–1597, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. M. A. Mazorra-Manzano, J. M. Moreno-Hernández, J. C. Ramírez-Suarez, M. D. J. Torres-Llanez, A. F. González-Córdova, and B. Vallejo-Córdoba, “Sour orange Citrus aurantium L. flowers: a new vegetable source of milk-clotting proteases,” LWT—Food Science and Technology, vol. 54, no. 2, pp. 325–330, 2013. View at Publisher · View at Google Scholar · View at Scopus
  15. H. Khan, M. Subhan, S. Mehmood, M. F. Durrani, S. Abbas, and S. Khan, “Purification and characterization of serine protease from seeds of Holarrhena antidysenterica,” Biotechnology, vol. 7, no. 1, pp. 94–99, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. M. Volpicella, F. D. Leo, M. Sciancalepore et al., “Identification and characterization of protease inhibitors in Diplotaxis species,” Plant Physiology and Biochemistry, vol. 47, pp. 175–180, 2009. View at Google Scholar
  17. T.-E. Chen, D.-J. Huang, and Y.-H. Lin, “Isolation and characterization of a serine protease from the storage roots of sweet potato (Ipomoea batatas [L.] Lam),” Plant Science, vol. 166, no. 4, pp. 1019–1026, 2004. View at Publisher · View at Google Scholar · View at Scopus
  18. L. P. Moro, H. Cabral, D. N. Okamoto et al., “Characterization, subsite mapping and N-terminal sequence of miliin, a serine-protease isolated from the latex of Euphorbia milii,” Process Biochemistry, vol. 48, no. 4, pp. 633–637, 2013. View at Publisher · View at Google Scholar · View at Scopus
  19. I. A. Cavello, R. A. Hours, N. L. Rojas, and S. F. Cavalitto, “Purification and characterization of a keratinolytic serine protease from Purpureocillium lilacinum LPS #876,” Process Biochemistry, vol. 48, no. 5-6, pp. 972–978, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. G. K. Patel, A. A. Kawale, and A. K. Sharma, “Purification and physicochemical characterization of a serine protease with fibrinolytic activity from latex of a medicinal herb Euphorbia hirta,” Plant Physiology and Biochemistry, vol. 52, pp. 104–111, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. Z.-Y. Li, W. Youravong, and A. H-Kittikun, “Protein hydrolysis by protease isolated from tuna spleen by membrane filtration: a comparative study with commercial proteases,” LWT—Food Science and Technology, vol. 43, no. 1, pp. 166–172, 2010. View at Publisher · View at Google Scholar · View at Scopus
  22. P. Tripathi, R. Tomar, and M. V. Jagannadham, “Purification and biochemical characterisation of a novel protease streblin,” Food Chemistry, vol. 125, no. 3, pp. 1005–1012, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. M. S. Arruda, F. O. Silva, A. S. Egito et al., “New peptides obtained by hydrolysis of caseins from bovine milk by protease extracted from the latex Jacaratia corumbensis,” LWT—Food Science and Technology, vol. 49, no. 1, pp. 73–79, 2012. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Savchenko, C. Vieille, S. Kang, and J. G. Zeikus, “Pyrococcus furiosus α-amylase is stabilized by calcium and zinc,” Biochemistry, vol. 41, no. 19, pp. 6193–6201, 2002. View at Publisher · View at Google Scholar · View at Scopus
  25. A. Haddar, A. Sellami-Kamoun, N. Fakhfakh-Zouari, N. Hmidet, and M. Nasri, “Characterization of detergent stable and feather degrading serine proteases from Bacillus mojavensis A21,” Biochemical Engineering Journal, vol. 51, no. 1-2, pp. 53–63, 2010. View at Publisher · View at Google Scholar · View at Scopus