Effects of Crude Extracts from Medicinal Herbs Rhazya stricta and Zingiber officinale on Growth and Proliferation of Human Brain Cancer Cell Line In Vitro
CAERS and CFEZO treatments induced apoptotic cell death. (a) Flow cytometric analyses of apoptosis and necrosis using Annexin V-FITC/PI staining. Panels I, II, III, and IV represent cells treated with vehicle, CAERS (100 μg/mL), CFEZO (100 μg/mL), and combined treatment of CAERS (20 μg/mL) and CFEZO (20 μg/mL), respectively. Cells in left lower quadrants represent viable population (annexin V-negative and PI-negative); cells in right lower quadrants represent early apoptotic population (annexin V-positive, PI-negative); cells in right top quadrants represent late apoptotic population (annexin V-positive and PI-positive) and cells in left top quadrants represent necrotic population (annexin V-negative and PI-positive). (b) Microphotographs showing CAERS and CFEZO treatments induced morphological features of apoptosis in U251 cells. The cells were treated with the indicated concentrations of CAERS and/or CFEZO for 48 h. Then, the photographs were taken directly from culture plates using a phase contrast microscope. Magnification of micrographs was as follows: I: 20x; II: 40x; and III: 63x. (c) Toluidine blue-stained semithin sections. The cells were treated and stained with toluidine blue, as detailed in Section 2. Depicted results are representative for independent experiments with almost identical observations.