Detection of Herpes Simplex and Varicella-Zoster Virus in Clinical Specimens by Multiplex Real-Time PCR and Melting Curve Analysis

Table 2

Comparison of real-time PCR, PCR-RFLP, and virus culture for the detection of HSV-1, HSV-2, and VZV during the validation phase.

Virus	Real-time PCR		PCR-RFLP		Virus culture
	Positive	Negative	Positive	Negative	Positive	Negative
HSV-1	9	91	8	92	4	96
HSV-2	6	94	6	94	4	96
VZV	19	81	13	87	0	100

HSV: herpes simplex virus; VZV: varicella-zoster virus.