Protective Role of 5-Lipoxigenase during Leishmania infantum Infection Is Associated with Th17 Subset
Figure 6
The absence of 5-LO interferes with BMDC maturation and the release of innate cytokines induced by L. infantum through BLT1 receptor. The WT or BMDC was stimulated with L. infantum (5 : 1) (black bars), LPS (200 ng/mL) (hatched bars), or medium (white bars) for 24 h. The BMDCs were harvested and the costimulatory molecules expression such CD86 (a), MHC-II (b), and CD40 (c) was evaluated by flow cytometry. All analyses were performed for the population. The TNF (d), IL-23 (e), IL-1β (f), and IL-6 (g) levels were measured in the supernatant of the BMDC culture by ELISA assay. The data are expressed as the mean ± SEM and are representative of three independent experiments. compared with the medium; compared with the infected WT. compared with the infected WT. In some experiment, selective BLT1 leukotriene B4 receptor antagonist (10 μM) was or not added 12 h before L. infantum infection or LPS stimuli. The levels of TNF (h), IL-23 (i), and IL-6 (j) were determinate into supernatant 24 thereafter. compared with the medium; compared with L. infantum infection. compared with LPS stimuli.
